This might be a repeat of a previous topic, but I didn't see one in the search I did on the forum here.
I was made aware of an issue with my Orion G16 Color camera (Touptek ATR3CMOS16000KPA) where a 12 bit image stored in a 16 bit FITS file would not be scaled properly. That is, the 12 bits per pixel would only occupy the first 4096 brightness values and never go above that (16 bits have about 65,000 values). The way the ASCOM driver does it is scale each pixel value by multiplying it by 16 (in PixInisght this is equivalent to using the pixelmath expression $T * 16.0), and both N.I.N.A. and APT have now added a pixel scaling feature that gives native scaled 16-bit FITS files:
APT - aptforum.com/phpbb/viewtopic.php?t=5354
NINA - nighttime-imaging.eu/docs/master/site/tabs/options/equipment/
Before Scaling: